C

Confocal microscopy

This program allows a series of tests over illumination sources for Zeiss widefield and confocal microscopes running Zen Blue. It is possibe to test:

Short term stability: According to QUAREP-LiMi full test is completed in 5 minutes for each illumination line, testing powers every second. Long term stability: The QUAREP-LiMi protocol stablishes 2h tests per line, with measurement intervals of 30s. To allow this for multiple lines this program interleaves the tests for all lines every interval, allowing the full check to be completed in a single run. Linearity (response): To analyze the linearity -or response curve- of each light source the powers are measured for a series of set powers. These tests can be performed for the desired lines and for different test conditions.