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This program allows a series of tests over illumination sources for Zeiss widefield and confocal microscopes running Zen Blue. It is possibe to test:

Short term stability: According to QUAREP-LiMi full test is completed in 5 minutes for each illumination line, testing powers every second. Long term stability: The QUAREP-LiMi protocol stablishes 2h tests per line, with measurement intervals of 30s. To allow this for multiple lines this program interleaves the tests for all lines every interval, allowing the full check to be completed in a single run. Linearity (response): To analyze the linearity -or response curve- of each light source the powers are measured for a series of set powers. These tests can be performed for the desired lines and for different test conditions.

Quantification of HCR in situ signal speckles in overall area or per individual cell. Main goal is to replicate publication results of difference marker expression between left and right sides in frogs.

Le Groupe Wojtan's Frolic of Frenetic Friction Frenzy

Stand to hold a sample chamber of a Zeiss lightheet horizontally during preparation

Protects your calibration objective.

Storage platform to keep a galvo stage insert safe, when not in use

To build a linkage map for asian Artemia species (Artemia sinica and Artemia Kazakhstan) based on crosses information and RNA-seq markers.

1-day long course and hands-on session for image analysis and ImageJ fundamentals.

Cut guide to safely get samples out of the Beem capsules.

Workshop about FAIR principles, research data management (RDM) and good practices in figure generation by the Imaging and Optics Facility (IOF) team at ISTA.